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Superoxide design are thought of in the bloodstream and BALF by using the CMH twist probe. Blood otherwise BALF trials were gathered out of PBS- and Bleo-addressed rats and you may incubated instantaneously which have CMH. The fresh examples have been gone to live in the newest PTFE tubing and you may flash frozen, and you will EPR analysis are obtained during the 77 K. The fresh intensity of nitroxide (CM.) obtained inside bloodstream incubated that have CMH (0.2 mM) at the 37 degree to own ten min (Contour 4A).
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